猪胰高血糖素样肽-2对结肠炎小鼠肠道损伤的保护作用研究

doi:10.11843/j.issn.0366-6964.2014.06.021

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (6): 1011-1017.doi: 10.11843/j.issn.0366-6964.2014.06.021

猪胰高血糖素样肽-2对结肠炎小鼠肠道损伤的保护作用研究

齐珂珂，吴杰，徐子伟^*

（浙江省农业科学院畜牧兽医研究所，杭州 310021）

收稿日期:2013-12-10 出版日期:2014-06-23 发布日期:2014-06-23
通讯作者: 徐子伟，研究员，博士生导师，E-mail：zjsnkyxzw@163.com
作者简介:齐珂珂（1981-），女，河南偃师人，博士，助理研究员，主要从事畜禽健康养殖的研究，E-mail：nkyqkk@163.com
基金资助:
现代农业产业技术体系（CARS-36）；国家青年科学基金项目（C170105/31101724）

The Therapeutic Effect of Porcine Glucagon-like Peptide-2 in a Murine Model of Experimental Colitis

QI Ke-ke，WU Jie，XU Zi-wei^*

(Institute of Animal Science，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China)

Received:2013-12-10 Online:2014-06-23 Published:2014-06-23

摘要/Abstract

摘要：

猪胰高血糖素样肽-2 （porcine glucagon-like peptide-2，pGLP-2）能够特异性地促进肠黏膜生长与损伤后修复，本试验研究了葡聚糖硫酸钠（dextran sulfate sodium，DSS）致小鼠结肠炎模型下pGLP-2对肠道损伤的修复效果，为其治疗各种因素引起的仔猪肠道损伤和功能紊乱提供了试验依据。18只BALB/c小鼠随机分为3组，DSS组小鼠饮用3% DSS建立小鼠结肠炎模型，DSS+pGLP-2组饮用3% DSS且连续7 d腹膜外注射pGLP-2，饮水组小鼠正常饮水。试验期10 d。结果表明，与饮水组相比，DSS组小鼠体重、结肠紧密连接闭锁小带（zonula occludens-1，ZO-1）基因的相对表达量显著降低，注射pGLP-2能够显著抑制体重降低和增加ZO-1的表达量；与饮水组相比，DSS组显著增加了结肠炎性评分、髓过氧化物酶（myeloperoxidase，MPO）活性和白细胞介素-6（interleukin-6，IL-6）、白细胞介素-10（interleukin-10，IL-10）和干扰素-γ（interferon-γ，INF-γ）的相对表达量，注射pGLP-2极显著降低了结肠炎性评分、MPO活性和IL-6、IL-10和IFN-γ的表达量。结果提示，pGLP-2可抑制结肠炎小鼠的炎性病变，为其治疗肠道疾病提供了试验依据。

Abstract:

As an intestinal-specific trophic factor，porcine glucagon-like peptide-2 (pGLP-2) could stimulate intestinal mucosal growth and reduce the severity of injury specificity.In order to provide a potential therapeutic agent for intestinal dysfunction and damage，the protective effects of pGLP-2 for colonic injury were measured in dextran sulfate sodium (DSS)-induced colitis in mice.Eighteen BALB/c mice were randomly assigned to three treatments for 10 days，mice of DSS group were fed 3% (w/v) DSS through their drinking water for 10 days from first day of experiment，mice of DSS+pGLP-2 group were extraperitoneally injected with pGLP-2 daily from day 3 to day 9 and drink 3% DSS.The water group received water normally.Results were as follows：DSS-induced colitis clearly decreased body weight and relative expression of zonula occludens-1 in the colon，which was significantly reduced by treatments with 3% DSS-pGLP-2.Administration of pGLP-2 to DSS-mice preserved normal colonic mucosal architecture and significantly decreased myeloperoxidase activity，the relative expression of interleukin-6，interleukin-10 and interferon-γ which induced by DSS.These results showed that pGLP-2 could reduce the severity of colonic injury in murine colitis.The potencies of this product highlight its potential as therapeutic agent for intestinal diseases in piglet.

中图分类号:

S856.4

齐珂珂，吴杰，徐子伟. 猪胰高血糖素样肽-2对结肠炎小鼠肠道损伤的保护作用研究[J]. 畜牧兽医学报, 2014, 45(6): 1011-1017.

QI Ke-ke，WU Jie，XU Zi-wei. The Therapeutic Effect of Porcine Glucagon-like Peptide-2 in a Murine Model of Experimental Colitis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 1011-1017.

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猪胰高血糖素样肽-2对结肠炎小鼠肠道损伤的保护作用研究

The Therapeutic Effect of Porcine Glucagon-like Peptide-2 in a Murine Model of Experimental Colitis

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